Free-labeled nanoclay intracellular uptake tracking by confocal Raman imaging

Laponite is a nanoplatform that has been successfully used as new biomaterial for drug delivery, tissue engineering and bioimaging at the nanoscale. In general, deep knowledge of mechanism interaction nanomaterial with biological components in physiological environment highly desirable properly characterizing its therapeutic efficacy toxicology. Up to know, use fluorescent dyes labelling both, cell components, requirement characterize uptake visualize entrance into cytosol nucleus. The fluorophores usually perturb medium can interfere interaction. A Raman imaging methodology track internalization nanoparticles J774 macrophages line cells presented this work. combination spectroscopy confocal microscopy provides direct information about localization nanoparticle cell, through unique vibrational fingerprint without or adding dyes, taking advantage fact molecules bands be clearly differentiated.